علوم زیستی دریا
negin derakhshesh; Abdolali Movahedinia; n s; m h; v b
Abstract
Liver is one of the most important organs in vertebrates that have important roles in detoxifying. The main purpose of the present study was developing appropriate methodology for the primary cultivation of hepatic cells from orange-spotted Grouper, Epinephelus coioides. In present study, hepatocytes ...
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Liver is one of the most important organs in vertebrates that have important roles in detoxifying. The main purpose of the present study was developing appropriate methodology for the primary cultivation of hepatic cells from orange-spotted Grouper, Epinephelus coioides. In present study, hepatocytes were isolated from five grouper individuals. Initially, the fish wiped with 70% ethanol. Liver were removed and cut into small pieces with scissors and hepatocytes were disconnected using different enzymatic digestion with collagenase (Type 1 and 4) and trypsin and additional nutrient materials in culturing mediums. Then, cells were cultured for 2 weeks in Lebowitz L-15 under 3 methods: 1. Using enzymatic digestion by trypsin, 2. Using enzymatic digestion by collagenase (type 1 and 4) and 3. Using nutrients and additives was cultured. Finally, effects of different incubation temperature (20, 25, 28, 30 and 32 degree of Celsius) and Bovine serum content (0, 10 and 20% and 20%+ITS) on cell growth were estimated. According to the results, digestion by collagenase type 4, resulted in more cell colonization and growth in comparison with other methods.In conclusion, the best culture method for primary hepatocyte from orange-spotted Grouper, Epinephelus coioides., was using ITS+20%FBS under 30 degree of Celsius incubation temprature.
علوم زیستی دریا
Sima Sarmadian; Alireza Safahieh; Hossein Zolgharnein; Bita Archangi; Mahmood Hashemi Tabar
Abstract
Heavy metals in high concentrations in the environment, is caused serious damage in metabolic, physiologic and structural organisms. Cadmium as the second most toxic metal in marine environments is considered. Bivalvia especially oysters are Suitable bioindicators due to its high filtration rates, immobility ...
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Heavy metals in high concentrations in the environment, is caused serious damage in metabolic, physiologic and structural organisms. Cadmium as the second most toxic metal in marine environments is considered. Bivalvia especially oysters are Suitable bioindicators due to its high filtration rates, immobility and lack of regulatory systems for removing of heavy metals. Crassostrea sp. is new species has wide distribution in Bandar Emam Khomeini. This species is the best for using toxicity testing to determine the effects of heavy metal pollutants in the environment. The aim this study is determination of Medium Lethal Concentration (LC5096h), Maximum Allowable Toxicant Concentration (MATC) and Lowest Observed Effect Concentration (LOEC) of Cadmium in Crassostrea sp. oyster. Fifty oysters (5.3±0.76 gr) were collected from Bandar Emam Khomaini and transported to laboratory. After Acclimatization (for 7 days), the 96-h LC50 tests were conducted (static Method) according to standard instruction O.E.C.D. The 96 h LC50, NOEC and LOEC were 15.8, 1.58 and 2.9 mg/l respectively. The LC50 correlation whit in 24 h and 96 h were showed Linear equation y=-0.4225x+54.35.